Chromatography Separation Factor Calculator
Calculate IUPAC separation factor α for two retained peaks.
α = k2 / k1 = (tR2 − tM) / (tR1 − tM)
Use a common tM and time unit with tR2 > tR1 > tM ≥ 0. IUPAC maps the familiar selectivity factor term to separation factor. At tM = 0, α remains defined but k1 and k2 cannot be computed.
All calculations run locally in your browser.
Original explanation based on IUPAC Gold Book terminology; attribution: IUPAC, Gold Book. Reference material: CC BY-SA 4.0. No source passages or figures are reproduced. License: CC BY-SA 4.0
A QUICK WALKTHROUGH
How to use this tool
- Enter the values in one common time unit, then calculate.
- Use a common tM and time unit with tR2 > tR1 > tM ≥ 0. IUPAC maps the familiar selectivity factor term to separation factor. At tM = 0, α remains defined but k1 and k2 cannot be computed.
Formula
α = k2 / k1 = (tR2 − tM) / (tR1 − tM)
Input basis
Use a common tM and time unit with tR2 > tR1 > tM ≥ 0. IUPAC maps the familiar selectivity factor term to separation factor. At tM = 0, α remains defined but k1 and k2 cannot be computed.
Processing and attribution
All calculations run locally in your browser. Original explanation based on IUPAC Gold Book terminology; attribution: IUPAC, Gold Book. Reference material: CC BY-SA 4.0. No source passages or figures are reproduced. https://goldbook.iupac.org/terms/view/S05614
GOOD TO KNOW
Common questions
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All calculations run locally in your browser.