Michaelis–Menten Calculator
Use v = Vmax[S]/(Km + [S]) for a single-substrate Michaelis–Menten model.
This is an initial-rate, single-substrate model. It assumes the Michaelis–Menten relationship applies under the assay conditions; it does not fit experimental data or account for cooperative kinetics, inhibitors, multiple substrates, or changing substrate during a reaction.
v = Vmax[S] / (Km + [S])
Initial velocity v
Sources: NCBI Assay Guidance Manual — Basics of Enzymatic Assays for HTS
A QUICK WALKTHROUGH
How to use this tool
- Enter Vmax and substrate concentration [S].
- Enter Km in the same concentration units as [S].
- Calculate the modeled initial velocity.
Model and limits
This is an initial-rate, single-substrate model. It assumes the Michaelis–Menten relationship applies under the assay conditions; it does not fit experimental data or account for cooperative kinetics, inhibitors, multiple substrates, or changing substrate during a reaction.
GOOD TO KNOW
Common questions
Does this fit enzyme assay data?
No. It evaluates one supplied point in the Michaelis–Menten equation. Use initial-rate measurements and a suitable fit to estimate kinetic parameters from experimental data.